Journal: International Dental Journal

Article Title: Hypoxia-Inducible Factor-1α Stabilization Alleviates Hypoxia-Induced Temporomandibular Joint Osteoarthritis by Activating MDM2 to Suppression of p53 Signalling

doi: 10.1016/j.identj.2026.109805

Figure Lengend Snippet: DMOG-mediated HIF-1α stabilization exerts a protective effect by inhibiting p53 signalling transduction. (A) Volcano plot of DEGs in chondrocytes treated with or without DMOG ( P < .05, |fold change| > 2) under hypoxic conditions. (B) KEGG pathway enrichment analysis of DEGs. (C) Representative immunohistochemical images of p53 in condylar chondrocytes (scale bar: 100 μm) and quantitative analysis of p53-positive area ( n = 6). (D and E) Western blot and quantitative analysis of HIF-1α, p53, p21, and BAX protein levels in chondrocytes under hypoxia and hypoxia + DMOG conditions. (F and G) Western blot images and quantitative analysis of iNOS, HIF-1α, MMP13, p53, p21, BAX, and TNF-α protein levels in chondrocytes under hypoxia, hypoxia + DMOG, and hypoxia + DMOG + NSC-207895 (HX + DMOG + NSC-207895) conditions ( n = 3). (H) Immunofluorescence staining images and quantitative analysis of IL-6 and MMP3 in chondrocytes treated with DMOG and NSC-207895 under hypoxia ( n = 3). Scale bar: 50 μm. Data are presented as mean ± SEM. Statistical significance was determined by unpaired Student’s t test (for pairwise comparisons) or one-way ANOVA (for multigroup comparisons), as appropriate. ns, no significance, * P < .05, ** P < .01, *** P < .001, **** P < .0001.

Article Snippet: The membranes were blocked with 5% nonfat milk and incubated with the following primary antibodies: HIF-1α (1:1000; Abcam), p53 (1:1000; ABclonal), TNF-α (1:1000; Huabio), MMP13 (1:1000; Abcam), iNOS (1:1000; Servicebio), BAX (1:1000; Proteintech), p21 (1:1000; Abcam), MDM2 (1:1000; Immunoway), and α-Tubulin (1:5000; Servicebio).

Techniques: Transduction, Immunohistochemical staining, Western Blot, Immunofluorescence, Staining

Journal: International Dental Journal

Article Title: Hypoxia-Inducible Factor-1α Stabilization Alleviates Hypoxia-Induced Temporomandibular Joint Osteoarthritis by Activating MDM2 to Suppression of p53 Signalling

doi: 10.1016/j.identj.2026.109805

Figure Lengend Snippet: Stabilized HIF-1α transcriptionally activates Mdm2 to promote p53 ubiquitin-proteasomal degradation in hypoxic condylar. (A) Co-IP assay detecting endogenous p53 ubiquitination in hypoxic chondrocytes treated with or without 1 mM DMOG. (B and C) qRT-PCR analysis of Tp53 and Mdm2 mRNA expression in chondrocytes cultured under hypoxia with or without DMOG intervention. (D) Reciprocal Co-IP assay for detecting physical interaction between MDM2 and p53 in hypoxic chondrocytes with or without DMOG treatment. (E) Western blot analysis of p53 protein abundance in hypoxic chondrocytes incubated with DMOG in the presence or absence of 10 μM MG132. (F) JASPAR database-based in silico prediction of potential HIF-1α binding sites on rat Mdm2 promoter. (G) ChIP-qPCR assay measuring HIF-1α occupancy on the Mdm2 promoter in hypoxic chondrocytes with or without DMOG. (H) Schematic diagram. Data are presented as mean ± SEM ( n = 3 per group). Statistical significance was determined by unpaired Student’s t test. ** P < .01, *** P < .001.

Article Snippet: The membranes were blocked with 5% nonfat milk and incubated with the following primary antibodies: HIF-1α (1:1000; Abcam), p53 (1:1000; ABclonal), TNF-α (1:1000; Huabio), MMP13 (1:1000; Abcam), iNOS (1:1000; Servicebio), BAX (1:1000; Proteintech), p21 (1:1000; Abcam), MDM2 (1:1000; Immunoway), and α-Tubulin (1:5000; Servicebio).

Techniques: Ubiquitin Proteomics, Co-Immunoprecipitation Assay, Quantitative RT-PCR, Expressing, Cell Culture, Western Blot, Quantitative Proteomics, Incubation, In Silico, Binding Assay, ChIP-qPCR